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automated synthesizer (applied biosystem 430a  (Thermo Fisher)


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    Structured Review

    Thermo Fisher automated synthesizer (applied biosystem 430a
    Automated Synthesizer (Applied Biosystem 430a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/automated+synthesizer+(applied+430a/us10040869-209-23-24
    Average 90 stars, based on 1 article reviews
    automated synthesizer (applied biosystem 430a - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Targeting the Two Oncogenic Functional Sites of the HPV E6 Oncoprotein with a High-Affinity Bivalent Ligand
    Article Snippet: The LxxLL (e6ap) and E6 PBM (e6ct) peptides were synthesized in the peptide synthesis unit of IGBMC (Illkirch, France) through Fmoc-chemistry with an automated solid phase peptide synthesizer Applied Biosystems 430A.

    Article Title: Tumor vasculature-targeted 10 B delivery by an Annexin A1-binding peptide boosts effects of boron neutron capture therapy
    Article Snippet: Automated peptide synthesis by Boc SPPS was performed on an ABI 430A peptide synthesizer (Applied Biosystems, CA, USA).

    Synthesized:

    Article Title: Disorder-to-order transition of MAGI-1 PDZ1 C-terminal extension upon peptide binding: thermodynamic and dynamic insights.
    Article Snippet: PDZ domains are highly abundant protein−protein interaction modules commonly found in multidomain scaffold proteins.. The PDZ1 domain of MAGI-1, a protein present at cellular tight junctions that contains six PDZ domains, is targeted by the E6 oncoprotein of the high-risk human papilloma virus.. Thermodynamic and dynamic studies using complementary isothermal titration calorimetry and nuclear magnetic resonance (NMR) N heteronuclear relaxation measurements were conducted at different temperatures to decipher the molecular mechanism of this interaction.

    Article Title: Specificity of the HIV-1 Protease on Substrates Representing the Cleavage Site in the Proximal Zinc-Finger of HIV-1 Nucleocapsid Protein
    Article Snippet: .. Oligopeptides were synthesized by solid-phase peptide synthesis on a Model 430A automated peptide synthesizer (Applied Biosystems, Inc., Foster City, CA, USA) using t-butoxycarbonyl (BOC) or 9-fluorenylmethyloxy-carbonyl chemistry and were purified by reversed-phase HPLC. .. The amino acid composition of the peptides was determined with a Beckman 6300 amino acid analyzer.

    Automated:

    Article Title: Disorder-to-order transition of MAGI-1 PDZ1 C-terminal extension upon peptide binding: thermodynamic and dynamic insights.
    Article Snippet: PDZ domains are highly abundant protein−protein interaction modules commonly found in multidomain scaffold proteins.. The PDZ1 domain of MAGI-1, a protein present at cellular tight junctions that contains six PDZ domains, is targeted by the E6 oncoprotein of the high-risk human papilloma virus.. Thermodynamic and dynamic studies using complementary isothermal titration calorimetry and nuclear magnetic resonance (NMR) N heteronuclear relaxation measurements were conducted at different temperatures to decipher the molecular mechanism of this interaction.

    Purification:

    Article Title:
    Article Snippet: .. The resin 3 can then be loaded into an automated peptide synthesizer (Applied Biosystems 430A) and the peptides elongated using standard solid phase procedures (5). c) The N-α-Boc group is removed with a solution of 45% trifluoroacetic acid in CH2Cl2. d) Subsequent Boc-protected amino acids (5 eq.) are preactivated using benzotriazol-1-yl-oxy-tris-(dimethylamino) phosphonium hexafluorophosphate (BOP, 4 eq.) and N-methylmorpholine (NMM, 10 eq.) in DMA and coupled for 1-2 h. e) The final N-α-Boc group is removed (TFA/CH2Cl2) to afford 4 and the isonicotinyl (iNOC) protecting group is introduced as described previously (4) via stirring with of 4-isonicotinyl-2-4-dinitrophenyl carbonate (3 eq.) and NMM (6 eq.) in DMA at 25° C. for 24 h. f) Cleavage and deprotection of the peptide via treatment with anhydrous HF (5% anisole/5% ethylmethyl sulfide) at 0° C. for 1 h. affords the iNOC-protected, glycolate-lys-amide activated peptide 5 which is purified by reverse phase C18 HPLC (CH3CN/H2O gradient, 0.1% TFA). ..

    Article Title: Monoclonal antibody to human thrombopoietin
    Article Snippet: .. The resin 3 can then be loaded into an automated peptide synthesizer (Applied Biosystems 430A) and the peptides elongated using standard solid phase procedures (5). c) The N-α-Boc group is removed with a solution of 45% trifluoroacetic acid in CH2Cl2. d) Subsequent Boc-protected amino acids (5 eq.) are preactivated using benzotriazol-1-yl-oxy-tris-(dimethylamino) phosphonium hexafluorophosphate (BOP, 4 eq.) and N-methylmorpholine (NMM, 10 eq.) in DMA and coupled for 1-2 h. e) The final N-α-Boc group is removed (TFA/CH2Cl2) to afford 4 and the isonicotinyl (iNOC) protecting group is introduced as described previously (4) via stirring with of 4-isonicotinyl-2-4-dinitrophenyl carbonate (3 eq.) and NMM (6 eq.) in DMA at 25° C. for 24 h. f) Cleavage and deprotection f t e peptide via treatment with anhydrous HF (5% anisole/5% ethylmethyl sulfide) at 0° C. for 1 h. affords the iNOC-protected, glycolate-lys-amide activated peptide 5 which is purified by reverse phase C18 HPLC(CH3CN/H2O gradient, 0.1% TFA). ..

    Article Title: Specificity of the HIV-1 Protease on Substrates Representing the Cleavage Site in the Proximal Zinc-Finger of HIV-1 Nucleocapsid Protein
    Article Snippet: .. Oligopeptides were synthesized by solid-phase peptide synthesis on a Model 430A automated peptide synthesizer (Applied Biosystems, Inc., Foster City, CA, USA) using t-butoxycarbonyl (BOC) or 9-fluorenylmethyloxy-carbonyl chemistry and were purified by reversed-phase HPLC. .. The amino acid composition of the peptides was determined with a Beckman 6300 amino acid analyzer.

    High Performance Liquid Chromatography:

    Article Title:
    Article Snippet: .. The resin 3 can then be loaded into an automated peptide synthesizer (Applied Biosystems 430A) and the peptides elongated using standard solid phase procedures (5). c) The N-α-Boc group is removed with a solution of 45% trifluoroacetic acid in CH2Cl2. d) Subsequent Boc-protected amino acids (5 eq.) are preactivated using benzotriazol-1-yl-oxy-tris-(dimethylamino) phosphonium hexafluorophosphate (BOP, 4 eq.) and N-methylmorpholine (NMM, 10 eq.) in DMA and coupled for 1-2 h. e) The final N-α-Boc group is removed (TFA/CH2Cl2) to afford 4 and the isonicotinyl (iNOC) protecting group is introduced as described previously (4) via stirring with of 4-isonicotinyl-2-4-dinitrophenyl carbonate (3 eq.) and NMM (6 eq.) in DMA at 25° C. for 24 h. f) Cleavage and deprotection of the peptide via treatment with anhydrous HF (5% anisole/5% ethylmethyl sulfide) at 0° C. for 1 h. affords the iNOC-protected, glycolate-lys-amide activated peptide 5 which is purified by reverse phase C18 HPLC (CH3CN/H2O gradient, 0.1% TFA). ..

    Article Title: Monoclonal antibody to human thrombopoietin
    Article Snippet: .. The resin 3 can then be loaded into an automated peptide synthesizer (Applied Biosystems 430A) and the peptides elongated using standard solid phase procedures (5). c) The N-α-Boc group is removed with a solution of 45% trifluoroacetic acid in CH2Cl2. d) Subsequent Boc-protected amino acids (5 eq.) are preactivated using benzotriazol-1-yl-oxy-tris-(dimethylamino) phosphonium hexafluorophosphate (BOP, 4 eq.) and N-methylmorpholine (NMM, 10 eq.) in DMA and coupled for 1-2 h. e) The final N-α-Boc group is removed (TFA/CH2Cl2) to afford 4 and the isonicotinyl (iNOC) protecting group is introduced as described previously (4) via stirring with of 4-isonicotinyl-2-4-dinitrophenyl carbonate (3 eq.) and NMM (6 eq.) in DMA at 25° C. for 24 h. f) Cleavage and deprotection f t e peptide via treatment with anhydrous HF (5% anisole/5% ethylmethyl sulfide) at 0° C. for 1 h. affords the iNOC-protected, glycolate-lys-amide activated peptide 5 which is purified by reverse phase C18 HPLC(CH3CN/H2O gradient, 0.1% TFA). ..

    Article Title: Specificity of the HIV-1 Protease on Substrates Representing the Cleavage Site in the Proximal Zinc-Finger of HIV-1 Nucleocapsid Protein
    Article Snippet: .. Oligopeptides were synthesized by solid-phase peptide synthesis on a Model 430A automated peptide synthesizer (Applied Biosystems, Inc., Foster City, CA, USA) using t-butoxycarbonyl (BOC) or 9-fluorenylmethyloxy-carbonyl chemistry and were purified by reversed-phase HPLC. .. The amino acid composition of the peptides was determined with a Beckman 6300 amino acid analyzer.



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